RNA

Part:BBa_K3001012:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-12)


Lwa crRNA targeting GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A double repeat stem loop is present to allow interactions with Cas13a Lwa. The target sequence has to be specific for GPF to minimize off-target effects.


Source

Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.

References